Composite

Part:BBa_K209065

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-12)

PTEN-linker-SHIP1

Dictyostelium PTEN (a 3' phospahatase) localizing to the back of cells, binding to PI(4,5)P2 fused to the catalytic domain of SHIP1 (a 5' phosphatase) acting on PI(3,4,5)P3

Documentation and Characterization

for details please see the UCSF team 2009 Wiki: [http://2009.igem.org/Matrix_of_Fusion_Constructs]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
    Illegal XhoI site found at 2791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1138


[edit]
Categories
Parameters
n/aPTEN-linker-SHIP1